|LETTER TO EDITOR
|Year : 2015 | Volume
| Issue : 1 | Page : 65
Antibacterial action of herbal agents
Vasudev N Ballal1, Jothi Varghese2
1 Department of Conservative Dentistry and Endodontics, Manipal College of Dental Sciences, Manipal University, Manipal, Karnataka, India
2 Department of 1Periodontology, Manipal College of Dental Sciences, Manipal University, Manipal, Karnataka, India
|Date of Web Publication||12-Jan-2015|
Vasudev N Ballal
Department of Conservative Dentistry and Endodontics, Manipal College of Dental Sciences, Manipal University, Manipal - 576 104, Karnataka
Source of Support: None, Conflict of Interest: None
|How to cite this article:|
Ballal VN, Varghese J. Antibacterial action of herbal agents. Saudi Endod J 2015;5:65
I read with great interest the article entitled "Comparison of antimicrobial efficacy of Propolis, Morindacitrifolia, Azadirachtaindica, triphala, green tea polyphenols, and 5.25% sodium hypochlorite against Enterococcus faecalis biofilm" by Garg P et al., which has been published in your esteemed journal (Saudi Endodontic Journal 2014; 4 (3):122-7). It was a good clinically relevant study, comparing the antibacterial effect of various herbal agents against Enterococcus faecalis. I wanted to share few of my thoughts regarding this manuscript.
- How were the teeth samples disinfected before using them for the canal preparation?
- The authors have grounded the root canal surface to make it flat. Hence, smear layer has been created during this procedure. However, no chelating agent has been used to remove this smear layer. Also, it has been demonstrated that E. faecalis has the ability to invade the dentinal tubules to varying depth.  Hence, by removing the smear layer, authors could have tested the efficacy of the test agents in eliminating E. faecalis which would have penetrated into the dentinal tubules
- The methodology which the authors have followed for the biofilm formation does not simulate the clinical scenario. Instead of sectioning the teeth vertically and forming the biofilm on the exposed root canal surface, the biofilm could have been developed inside the intact root canal and then the test agents could have been irrigated and tested for their effectiveness 
- Two percent chlorhexidine gluconate solution could have been used as one of the control group since it is one of the commonly used endodontic irrigant and have demonstrated to be very effective against E. faecalis,
- How was the biofilm formation on the root canal dentin surface confirmed? A scanning electron microscope (SEM) or confocal laser scanning microscopic image could have been done to confirm the biofilm formation 
- Collection of the sample by paper points is not a reliable method. Instead, root canal dentin shavings or pulverization could have been used for sample collection. 
Hence, authors can incorporate all these above-mentioned parameters and perform further similar studies to appreciate for the better performance of the herbal agents as endodontic irrigants.
| References|| |
Figdor D, Davies JK, Sundqvist G. Starvation survival, growth, and recovery of Enterococcus faecalis
in human serum. Oral Microbiol Immunol 2003;18:234-9.
Xie Q, Johnson BR, Wenckus CS, Fayad MI, Wu CD. Efficacy of berberine, an antimicrobial plant alkaloid, as an endodontic irrigant against a mixed-culture biofilm in an in vitro
tooth model. J Endod 2012;38:1114-7.
Kandaswamy D, Venkateshbabu N, Gogulnath D, Kindo AJ. Dentinal tubule disinfection with 2% chlorhexidine gel, propolis, morindacitrifolia juice, 2% povidone iodine, and calcium hydroxide. Int Endod J 2010;43:419-23.
Lee Y, Han SH, Hong SH, Lee JK, Ji H, Kum KY. Antimicrobial efficacy of a polymeric chlorhexidine release device using in vitro
model of Enterococcus faecalis
dentinal tubule infection. J Endod 2008;34:855-8.
Arias-Moliz MT, Ferrer Luque CM, Espigares-Garcia M, Baca P. Enterococcus faecalis
biofilms eradication by root canal irrigants. J Endod 2009;35:711-4.
Tran KT, Torabinejad M, Shabahang S, Retamozo B, Aprecio RM, Chen JW. Comparison of efficacy of pulverization and sterile paper point techniques for sampling root canals. J Endod 2013;39:1057-9.