Home Print this page Email this page Users Online: 203
Home About us Editorial board Search Ahead of print Current issue Archives Submit article Instructions Subscribe Contacts Login 
Year : 2022  |  Volume : 12  |  Issue : 1  |  Page : 56-60

Antibacterial efficacy of bioceramic root canal sealers against planktonic Enterococcus faecalis after different contact and setting time: An in vitro study

1 Department of Endodontics and Restorative Dentistry, Dental Polyclinic Split, Split, Croatia
2 Department of Medical Chemistry and Biochemistry, School of Medicine, University of Split, Split, Croatia
3 Study Programme of Dental Medicine, School of Dental Medicine, University of Split, Split, Croatia
4 Department of Endodontics and Restorative Dentistry, School of Dental Medicine, University of Zagreb, Zagreb, Croatia

Correspondence Address:
Marija Simundic Munitic
Department of Endodontics and Restorative Dentistry, Dental Polyclinic Split, A.G. Matosa 2, 21000 Split
Login to access the Email id

Source of Support: None, Conflict of Interest: None

DOI: 10.4103/sej.sej_12_21

Rights and Permissions

Introduction: Bioceramic root canal sealers are the newest generation of root canal sealers. Τhere are contradictory results in the literature about their antimicrobial activity. The aim of the study was to evaluate the antimicrobial efficacy of four root canal sealers against Enterococcus faecalis. Materials and Methods: Four root canal sealers were used in order to examine antibacterial efficacy: TotalFill, BioRoot Root Canal Sealer, mineral trioxide aggregate (MTA) Fillapex, and AH Plus. The bacterial suspension was placed on a freshly mixed sealer, one or 3 days set sealer in vertically held microtiter plates. After incubation of 2, 5, 20, and 60 min in 100% humidity at 37 C, Trypticase Soy Broth was added to each well and mixed. Then, bacterial suspension from each well was transferred, serially diluted, and placed on Mitis salivarius agar plates. After incubation, colony-forming units were counted. All experiments were performed in triplicate. The outcomes of antimicrobial properties of tested materials were analyzed by one-way analysis of variance. Results: All bioceramic root canal sealers showed significantly better efficacy than the control group and epoxy resin sealer (P < 0.05). TotalFill presented the highest efficacy comparable with BioRoot RCS (P > 0.05), followed with MTA Fillapex (P < 0.05). Also, freshly mixed sealers showed comparable efficacy as sealers set for 3 days (P > 0.05), but better than sealers set for 1 day (P < 0.05). All sealers exhibited the highest efficacy after 20 min of contact time, independently of materials that were freshly mixed or set for 1 or 3 days. Conclusion: Bioceramic sealers have greater antimicrobial activity than commercially used epoxy resin sealer. These sealers exhibited the strongest efficacy at 20 min of contact time, independently of their setting condition.

Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)

 Article Access Statistics
    PDF Downloaded24    
    Comments [Add]    

Recommend this journal