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LETTER TO EDITOR
Year : 2023  |  Volume : 13  |  Issue : 1  |  Page : 118-119

Author's reply


Department of Surgery, Anesthesiology, and Radiology, Faculty of Veterinary Medicine, Cairo University, Giza, Giza Square, Egypt

Date of Web Publication11-Jan-2023

Correspondence Address:
Prof. Ashraf M Abu-Seida
Department of Surgery, Anesthesiology, and Radiology, Faculty of Veterinary Medicine, Cairo University, Giza, Giza Square
Egypt
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/1658-5984.367525

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How to cite this article:
Abu-Seida AM. Author's reply. Saudi Endod J 2023;13:118-9

How to cite this URL:
Abu-Seida AM. Author's reply. Saudi Endod J [serial online] 2023 [cited 2023 Feb 3];13:118-9. Available from: https://www.saudiendodj.com/text.asp?2023/13/1/118/367525

Dear Editor,

In reference to the comments sent by Professor Dr. Mahalaxmi Sekar, regarding our published article “Histological evaluation of the synergistic effect of chitosan and mineral trioxide aggregate (MTA) on mechanically exposed dental pulp following pulp capping in dogs' teeth,”[1] kindly find below our reply.

The current study focused on the histopathological assessment in terms of the quality of dentin bridge formation and the effect of a new modulation of MTA preparation on the repair capacity of exposed dental pulps.[1]

In our study, MTA was prepared with a modified form of its original liquid containing 10% wt chitosan. This technique was similarly used in a previous study but MTA was prepared with a modified form of its original liquid containing 10% wt calcium chloride and this combination was applied as a pulp capping material in dogs' teeth, taking into account that the pH of the calcium chloride solution is 4.0 to 6.0 at room temperature.[2] In our study, MTA powder (1 g) and the Chitosan solution were mixed in a 3:1 powder-to-liquid ratio. Once all powder particles were well incorporated into the chitosan solution using a sterile spatula, the mix was then transferred to the exposed pulpal floor by an amalgam carrier and slightly packed by a suitable size condense.[1]

Although assessing the physicochemical and microstructural changes following the addition of chitosan to MTA was not the scope of our study; however, if it has been assessed side to side with the biological outcomes, this would have certainly added a great value to the study. Your comment is highly meaningful to us and would be a gate for future research to accurately assess the physiochemical changes at a molecular level after the addition of chitosan to MTA. In a previous study, we also studied the influence of the addition of chitosan to MTA on the gene expression level of odontoblastic markers following pulp capping in a dog model.[3]

As regards, the comment about using 10 wt% concentration of chitosan, kindly note that there is almost no standardized specific weight-volume concentration for mixing chitosan with MTA up till now. For instance, 2% wt/vol chitosan was used in a previous study where 1 drop of chitosan was mixed manually along with the MTA sealer.[4]

Concerning the final restoration with glass ionomer over the 60 days' time period; many earlier workers used glass ionomer as a final restoration directly over the MTA for evaluation periods extending up to 2 years in humans and 3 months in dogs without reporting any adverse effects on the restoration seal or integrity.[5],[6],[7],[8]

Regarding the comment about not using the split-mouth design in the study, kindly note that the mouth of each dog was divided into experimental segments according to the pulp capping material used and the dogs were randomly assigned to three evaluation periods.

Finally, we would like to thank Professor Mahalaxmi Sekar for her valuable comments.

 
  References Top

1.
Emara RA, Abu-Seida AM, El Ashry SH. Histological evaluation of the synergistic effect of chitosan and mineral trioxide aggregate on mechanically exposed dental pulp following pulp capping in dogs' teeth. Saudi Endod J 2022;12:25-30.  Back to cited text no. 1
  [Full text]  
2.
Parirokh M, Asgary S, Eghbal MJ, Kakoei S, Samiee M. A comparative study of using a combination of calcium chloride and mineral trioxide aggregate as the pulp-capping agent on dogs' teeth. J Endod 2011;37:786-8.  Back to cited text no. 2
    
3.
El Ashry SH, Abu-Seida AM, Emara RA. The influence of addition of osteogenic supplements to mineral trioxide aggregate on the gene expression level of odontoblastic markers following pulp capping in dogs. Vet Arhiv 2016;86:685-97.  Back to cited text no. 3
    
4.
Pattanaik S, Jena A, Shashirekha G. In vitro comparative evaluation of antifungal efficacy of three endodontic sealers with and without incorporation of chitosan nanoparticles against Candida albicans. J Conserv Dent 2019;22:564-7.  Back to cited text no. 4
  [Full text]  
5.
Negm AM, Hassanien EE, Abu-Seida AM, Nagy MM. Biological evaluation of a new pulp capping material developed from Portland cement. Exp Toxicol Pathol 2017;69:115-22.  Back to cited text no. 5
    
6.
Saleh R, Nagi SM, Khallaf ME, Abd El-Alim SH, Zaazou MH, Abu-Seida AM, et al. In-vivo assessment of dentin bridge formation after using MTA and experimental propolis paste as direct pulp capping material. Res J Pharmaceut Biol Chem Sci 2016;7:1244-50.  Back to cited text no. 6
    
7.
Parinyaprom N, Nirunsittirat A, Chuveera P, Na Lampang S, Srisuwan T, Sastraruji T, et al. Outcomes of direct pulp capping by using either proroot mineral trioxide aggregate or biodentine in permanent teeth with carious pulp exposure in 6- to 18-year-old patients: A randomized controlled trial. J Endod 2018;44:341-8.  Back to cited text no. 7
    
8.
Katge FA, Patil DP. Comparative analysis of 2 calcium silicate-based cements (Biodentine and Mineral Trioxide Aggregate) as direct pulp-capping agent in young permanent molars: A split mouth study. J Endod 2017;43:507-13.  Back to cited text no. 8
    




 

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