|LETTER TO EDITOR
|Year : 2023 | Volume
| Issue : 1 | Page : 118-119
Ashraf M Abu-Seida
Department of Surgery, Anesthesiology, and Radiology, Faculty of Veterinary Medicine, Cairo University, Giza, Giza Square, Egypt
|Date of Web Publication||11-Jan-2023|
Prof. Ashraf M Abu-Seida
Department of Surgery, Anesthesiology, and Radiology, Faculty of Veterinary Medicine, Cairo University, Giza, Giza Square
Source of Support: None, Conflict of Interest: None
|How to cite this article:|
Abu-Seida AM. Author's reply. Saudi Endod J 2023;13:118-9
In reference to the comments sent by Professor Dr. Mahalaxmi Sekar, regarding our published article “Histological evaluation of the synergistic effect of chitosan and mineral trioxide aggregate (MTA) on mechanically exposed dental pulp following pulp capping in dogs' teeth,” kindly find below our reply.
The current study focused on the histopathological assessment in terms of the quality of dentin bridge formation and the effect of a new modulation of MTA preparation on the repair capacity of exposed dental pulps.
In our study, MTA was prepared with a modified form of its original liquid containing 10% wt chitosan. This technique was similarly used in a previous study but MTA was prepared with a modified form of its original liquid containing 10% wt calcium chloride and this combination was applied as a pulp capping material in dogs' teeth, taking into account that the pH of the calcium chloride solution is 4.0 to 6.0 at room temperature. In our study, MTA powder (1 g) and the Chitosan solution were mixed in a 3:1 powder-to-liquid ratio. Once all powder particles were well incorporated into the chitosan solution using a sterile spatula, the mix was then transferred to the exposed pulpal floor by an amalgam carrier and slightly packed by a suitable size condense.
Although assessing the physicochemical and microstructural changes following the addition of chitosan to MTA was not the scope of our study; however, if it has been assessed side to side with the biological outcomes, this would have certainly added a great value to the study. Your comment is highly meaningful to us and would be a gate for future research to accurately assess the physiochemical changes at a molecular level after the addition of chitosan to MTA. In a previous study, we also studied the influence of the addition of chitosan to MTA on the gene expression level of odontoblastic markers following pulp capping in a dog model.
As regards, the comment about using 10 wt% concentration of chitosan, kindly note that there is almost no standardized specific weight-volume concentration for mixing chitosan with MTA up till now. For instance, 2% wt/vol chitosan was used in a previous study where 1 drop of chitosan was mixed manually along with the MTA sealer.
Concerning the final restoration with glass ionomer over the 60 days' time period; many earlier workers used glass ionomer as a final restoration directly over the MTA for evaluation periods extending up to 2 years in humans and 3 months in dogs without reporting any adverse effects on the restoration seal or integrity.,,,
Regarding the comment about not using the split-mouth design in the study, kindly note that the mouth of each dog was divided into experimental segments according to the pulp capping material used and the dogs were randomly assigned to three evaluation periods.
Finally, we would like to thank Professor Mahalaxmi Sekar for her valuable comments.
| References|| |
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